Investigation of Drug Delivery of Rattle-Structured Gold Nanorod-Mesoporous Silica Nanoparticles Core–Shell as Curcumin Carrier and Their Effect on MCF۷ and ۴T۱ Cell Lines

Background and Objective: Curcumin is a polyphenolic anti-cancer and anti-inflammatory agent and can be used both orally and by injection. In this study, nanoparticles of silica mesopore- gold nano were synthesized as a new drug delivery system. For this aim, gold nanoparticles as a promising system for effective drug delivery of curcumin was used and nano gold were delivered to breast cancer cells . Materials and Methods: The synthetic nanoparticles characteristics were determined with different techniques such as transmission electron microscopy (TEM), nitrogen gas desorption, absorption analysis and dynamic light scattering techniques (DLS). The anti-cancer effects of this system were evaluated in cell culture, using MTT cytotoxicity test. The efficiency of this system was tested on human breast cancer (MCF-۷) and ۴T۱ cell lines. Results: The mean size of nano system was less than ۳۰ nm, similar to the data obtained from Dynamic light scattering technique. The amount of curcumin loading in nano- system was about ۸۰ percent. Isotherm - nitrogen adsorption AuNR@msio۲ and CUR @ AuNR @ msio۲ show that specific area of nano-Au @ msio۲ was about -۱۸۳۴ m۲g but by creating medication this particular area decrease to ۲۳۴ m۲g-۱. IC۵۰ for curcumin loaded mesoporous silica nanoparticles for MCF-۷ at the time of ۴۸ hours and ۴T۱ were ۱۹.۵ and ۱۴.۶ micro molar respectively. The survival rate of the cells after treatment with different concentrations did not change considerably in the nano structures which demonstrate the lack of toxicity of nanoparticles. Conclusion: The nanoparticle caused apoptosis in breast cancer cells more than free drug.    

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Keywords: Curcumin , Gold , Breast cancer , Drug delivery systems , Cell toxicity test