Investigation of Caspase ۹ Gene Expression and Apoptosis Induction after Photodynamic Therapy with Zinc Phthalocyanine in SW۸۷۲ Skin Cancer Cell Line
Publish Year: 1397
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_ZUMS-26-116_002
تاریخ نمایه سازی: 11 اردیبهشت 1400
Abstract:
Background and Objective: The treatment of cancer comes as a great challenge worldwide. Thus the development of effective therapies with minimal side effects is important. Photodynamic therapy is a non-invasive and new therapeutic approach for the treatment of cancer. Therefore, in this study, we evaluated the photodynamic effects of a light-sensitive compound, Zinc-phthalocyanine (ZnPc), on cell growth and the expression of caspase-۹ in SW۸۷۲ cell line.
Materials and Methods: For cytotoxic effects of photodynamic therapy with ZnPc, MTT assay was conducted. Quantifying the level of caspase-۹ gene expression was carried out with qRT-PCR. The statistical analysis was done by GraphPad Prism ۶ software.
Results: MTT assay results showed that photodynamic therapy with ZnPc significantly reduced cancer cell growth. The IC۵۰ for cell line SW۸۷۲ was ۰/۰۳۴ μg/ml in a ۲۴ J/cm۲ light source. qRT-PCR results showed that photodynamic therapy with ZnPc increased the gene expression of caspase-۹ in the treated group.
Conclusion: Photodynamic therapy with ZnPc resulted in decreased cell growth and induced apoptosis in a concentration-dependent manner in SW۸۷۲ cell lines. Based on the results, photodynamic therapy with ZnPc can be a powerful therapeutic approach for the treatment of skin cancer with minimal side effects.
Authors
محمدامین دوستوندی
Dept. of Genetics, Islamic Azad University, Tabriz Branch, Tabriz, Iran
فاطمه محمدنژاد
Dept. of Genetics, Islamic Azad University, Tabriz Branch, Tabriz, Iran
محمدرضا مشایخی
Dept. of Genetics, Islamic Azad University, Tabriz Branch, Tabriz, Iran
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