Genome-wide association study for post weaning weight traits in Lori- Bakhtiari sheep

Publish Year: 1399
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:

CIGS16_005

تاریخ نمایه سازی: 14 اردیبهشت 1400

Abstract:

Background and Aim: Postweaning body weights, are important economic traits, especially for meat type farm animals. This study was conducted to perform a genome-wide association study (GWAS) to detect quantitative trait loci associated with postweaning weight traits in Lori-Bakhtiari sheep.Methods: Body weight records, incloding ۶-month weight (۶MW), ۹-month weight (۹MW) and yearling weight (YW) were collected during ۱۹۸۹ to ۲۰۱۷ in the flock of Lori-Bakhtiari sheep at Shooli Breeding Station in Shahre-Kord, Iran. Estimates of breeding values (EBVs) for the studied traits were estimated by analysis of animal mixed models, using WOMBAT software. A total of ۱۳۲ animals were selected by two-tailed selection strategy, based on EBVs for body weight. Genomic DNA was extracted from whole blood samples, using 〖DNP 〗^TM Kit and then were genotyped using Illumina ۵۰k ovine SNP chip. De-regressed estimates of breeding values (dEBVs) were used as a pseudo-phenotypes. Quality control and GWAS analyses were performed using Plink ۱.۹۰ beta software. After quality control, a total of ۱۳۰ animals and ۴۱۳۲۳ SNPs were remained for further analyses.Results: Based on Bonferroni-adjusted p-values, ۳ SNPs, located on chromosomes ۱۰ and ۱۳ were significantly (p < ۰.۰۵) associated with the studed traits and accounted for ۰.۰۲ and ۰.۴۸% of total genetic variations of ۶MW and ۹MW, respectively. The identified SNPs were located within two genes, ATPase Phospholipid Transporting ۸A۲ (ATP۸A۲) and Plexin Domain Containing ۲ (PLXDC۲), both protein coding genes. Based on molecular and cellular functions, both detected genes could be considered as candidate genes for post weaning body weight traits.Conclusion: The detected QTLs and candidate genes in the present study could be subjected to more studied for construction of breeding programs for genetic improvement of growth performance in meat type sheep.

Authors

Mohammad almasi

Bu-Ali Sina University, Hamedan, Iran

pouya zamani

Bu-Ali Sina University, Hamedan, Iran

Seyed Ziaeddin Mirhoseini

Guilan University, Rasht, Iran

Mohammad Hussein Moradi

Arak University, Arak, Iran.