Anticancer Effect of Gemini Nanocurcumin in Colon Cancer Cell Lines

Background and Aim: Colorectal cancer is the third frequently diagnosed illness in the world. Curcumin is polyphenolic agent in turmeric plant, which has been demonstrated to have a strong anticancer effects. Yet, its low solubility and poor uptake restricts its application. We aimed to study the anticancer activity of Gemini Nanocurcumin (GNC) in colon cancer cell lines.Methods: The toxic effects of GNC on p۵۳ mutant/Wild-type colon cancer cell lines including HT-۲۹ and HCT۱۱۶ was studied through MTT, uptake kinetics, fluorescence microscopy, annexin V/FITC, and cell cycle assays. Also, qRT-PCR and Western blot were performed to evaluate p۵۳, p۲۱, BAX, Bcl-۲, and Noxa expression at both RNA and protein levels.Results: Our data revealed that , GNC increased the SubG۱ cells and induced G۲/M cell cycle arrest. In addition, Real-Time PCR and Western blot assays showed an increased expression of BAX, Noxa, p۲۱, and p۵۳ in a time/dose-dependent manner and a decreased expression of Bcl-۲ in desired colon cancer cells.Conclusion: Our study showed that GNC, can perform its anticancer effects by modifying the expression of apoptotic and proliferative genes in both p۵۳ wild-type and p۵۳ mutant colon cancer cell lines via different effective doses.

Keywords:

Gemini Nanocurcumin (GNC) , p۵۳ Mutant , Apoptosis , Colon Cancer