Farzaneh Teimouri - Department of Biotechnology Faculty of Biological Sciences and Technology Shahid Ashrafi Esfahani University,Isfahan, Iran.
Ali Mohammad Ahadi - Associate Professor, Department of Genetic, Faculty of Science, Shahrekord University. Shahrekord, Iran
Zahra Zamanzadeh - assistant professor Department of Biotechnology Faculty of Biological Sciences and Technology Shahid Ashrafi Esfahani University, Isfahan, Iran

Background and Aim: The concept of ‘lifestyle’ includes different factors such as nutrition, diet, psychological stress, physical activity, working habits, environmental pollutants, smoking and alcohol consumption. Several factors both inside and outside the body contribute to gene expression and the development of cancer. There is evidences that the lifestyle has significant effects on the pattern of epigenetic changes. The aim of this study was to investigate the effects of lifestyle on gene expression in cancer.Methods: Ten mail Balb /c mice were divided into control and treatment groups. Each group was fed for two months. Control group with standard rat diet and treatment group as ۵۰% standard diet and ۵۰% omega-۳ compounds. After two months, mice’s colon were separated and their RNA was extracted. Extracted RNA was used for cDNA synthesis and then Real Time PCR was done.Results: Result showed oncogenes were down regulated in treated mice than control group. Tumor suppressor genes showed increased expression in treated mice than control group.Conclusion: Therefor changes in lifestyle have a high impact on reducing the expression of oncogenes and increasing the tumor suppressor genes expression thus reducing the risk of cancer.

Lifestyle, colorectal cancer, gene expression, oncogene, tumor suppressor