Antigenic epitopes indentification of Vibrio cholerae surface proteins and designing an its expression cassette

Background and Aim: Vibrio cholerae, which has always been a threat to health in large parts of the world, is the origin of an acute intestinal infectious disease that dates back to ۱۸۱۷. Today, the disease is most commonly seen in third world countries that are poor in health. This bacterium belongs to the Vibrionaceae family, having two major serotypes, Ogawa and Inaba, and two classical biotopes, and Eltor. Vaccines have always been one of the best ways to prevent disease. With the advances in science and technology, vaccine production methods have undergone fundamental changes. Informatics has helped a lot. Immunoinformatic is a new way of designing vaccines that are far more efficient than ever before, using several important membrane proteins of this bacterium as a vaccine candidate in this study.Methods: After extracting linear and spatial epitopes of target proteins in multiple databases such as IEDB, LBtope, ABCpred, Ellipro, Discotope, and CBtope, according to the criteria used in vaccine design, we arrive at a polytope construct that after optimization And translating it to DNA can be expressed by designing an expression cassette to target the gene in the vector.Results: After bioinformatics analysis on ۶ membrane proteins, we reached ۳۵ suitable epitopes, which after final examination and considering their physicochemical properties and Space structure, we got ۲ epitopes for omp U, ۲ epitopes for omp W, ۳ epitopes for omp A, ۳ epitopes for omp K, ۲ epitopes for omp V and ۲ epitopes for omp C. Combining these epitopes together we created a standard polytope construct and expressed it by designing a specific expression cassette in the pET۲۱a vector.Conclusion: With significant advances in computer techniques in the field of biomedical and medical sciences such as vaccine design, a vaccine with the highest impact and least side effect can be developed for any alien agent in the shortest time and with the least cost.