Competition of miR-۴۵۱ in Breast Cancer using RT-Qpcr

Background and Aim: Breast cancer is the most common invasive cancer in females worldwide. It accounts for ۱۶% of all female cancers and ۲۲.۹% of invasive cancers in women. miR-۴۵۱ is the most down-regulated miRNAs in inflammatory breast carcinoma and able to enhance tamoxifen sensitive. The aim of this study was to compare the expression level of miR-۴۵۱ in normal and neoplastic samples from breast cancer patients using RT-qPCR.Methods: In the present case-control study, ۲۰ samples from patients with breast cancer tumor and ۲۰ patients from non-tumor were collected under the direct supervision of a pathologist specialist due to clinical presentation and laboratory findings. After extracting RNA from normal and tumor tissues, cDNA synthesis method according to the protocol and RT-qPCR were performed by SYBR®Premix Ex TaqTM II kit. miR-۴۵۱ expression levels was calculated using ∆∆CT. Data were analyzed using t-test.Results: The results of molecular method indicated that the mean relative expression levels of miR-۴۵۱ in tumor samples compared to normal was down-regulated. This variation expression of miR-۴۵۱ in tumor samples was about ۲.۱ fold less than normal samples.Conclusion: According to previous reports, miR-۴۵۱ may play an important role in breast cancer progression, and that the down-regulation of miR-۴۵۱ may serve as independent predictors for breast cancer.

Keywords:

Breast cancer , miRNAs , miR-۴۵۱ , RT-qPCR.