Molcular detection of G۱۶۹۱A in the factor ۵ Leiden and and its relation with TNF−α gene polymorphisms in abortion human.

Background and Aim: Abortion is the most common of pregnancy complications.In the pathogenesis abortion, Genetic factors are involved. In the pathogenesis of abortion as a multifactorial disease, genetic factors and environmental factors are related.Hereditary thrombophilia as one of the factors known to be involved in abortion and Factor ۵ Leiden (G۱۶۹۱A) is One of the most important factors is the hereditary coagulation thrombosis. In connection with this mutation and abortion, there are contradictions. In connection with this mutation and abortion, there are contradictions. Another factor in recurrent miscarriage fetal inflammatory factors is considered that TNFα is one of the most important pro-inflammatory Cytokines and played a key role in many infections and inflammatory diseases as well. Polymorphism TNFα Susceptibility to various diseases associated with chronic inflammation. Of course, this Polymorphism association with abortion caused by inflammation in Iran has been less studied. This study aimed to investigate the relationship between each of Polymorphisms G۱۶۹۱A and ۳۰۸- TNFα with abortion and the relationship of these two Polymorphisms, are at an increased risk in women with abortion was performed .Methods: In this case-control study, ۴۷ patients with a history of abortion and ۱۰۵ Genomic DNA and ۵۸ controls were selected. DNA extracted from the venous blood and then to determine the genotypes of polymorphisms G۱۶۹۱A and ۳۰۸- TNFα, were used ARMS - PCR and electrophoresis techniques were evaluated Results: In connection with the polymorphism G۱۶۹۱A,GG and GA genotype frequencies in controls, respectively ۹۵% and ۵% and ۸۷% and ۱۳% respectively in patient samples relationship between case and control groups was significant (P=۰/۰۵).About polymorphism ۳۰۸- TNF? genotype frequencies GA, GG and AA in the control samples, respectively ۵۴%, ۳۱% and ۱۵% in patient samples, ۱۹%, ۴۵%, ۳۶%, which is still a significant correlation between the control group and patients there (P = ۰/۰۰۰۰۱)results. But at the same Frequency, GA genotype G۱۶۹۱A mutation and polymorphism AA genotype of ۳۰۸- TNF?, there is a significant relationship between the two groups was not significant.Conclusion: In general it can be concluded that molecular analysis of mutations in Factor ۵ Leiden G۱۶۹۱A polymorphism and ۳۰۸- TNFα in early detection and early treatment is effective abortion.

Keywords:

Abortion , Polymorphism G۱۶۹۱A , Polymorphism TNFα ۳۰۸