Investigating the effect of Iranian rice bran extract on the expression of PPAR-alpha gene and morphological changes of adipose tissue cells in model mice

Background and Aim: Obesity increases the risk of various diseases, including cardiovascular disease, type ۲ diabetes, sleep apnea, certain types of cancer, osteoarthritis and depression. Different methods have introduced focused on preventing and treatment of obesity in various reports. In this study, We looked at changes in fatty tisssue and PPAR-alpha gene expression. Rice bran extract, which is introduced in traditional medicine as an inducer of fat burning, is examined.Methods: The three groups of seven males, Balb / C mice, included the control group with standard nutrition, the second group with standard nutrition and olive oil Gavage, which was used as the accessory for extraction, and the treatment group, which was feed with rice bran extract in addition to standard food. The mice were monitored for ۶ weeks. In the next step, RNA extraction was performed from liver and intestinal tissues of mice considering standard protocols and the expression of PPAR-alpha gene was investigated by Real-time RT_PCR method. Also, using Oil Red method, the adipose tissue of the three groups was examined and compared.Results: The results showed that the expression of PPAR-alpha gene in the treatment group was significantly higher than the control groups (CI: ۹۵%, P<۰.۰۵, Sig.=۰.۰۱۳ and FC=۸۱). Also, in the adipose tissue of the treatment group, despite the relative increase in the number of cells, the cell size was reduced by about half, which indicates a decrease in the tendency of adipose tissue to act as a storage cell.Conclusion: The results confirm the role of effective factors in rice bran extract on the expression of PPAR-alpha gene as one of the most important factors in increasing fat catabolism in the body

Keywords:

Rice bran extract , PPAR-alpha , adipose tissue , Catabolism