An association study of miR-۱۲۵b in breast cancer in Isfahan province of Iran

Background and Aim: Breast cancer is regarded as a heterogeneous and complicated disease which accounts as a major health problem worldwide. Invasion and metastasis with strong impacts on prognosis and survival of breast cancer patients could be regulated by so many genes and also micro RNAs (miRNAs). Literature reviews have shown that miR-۱۲۵b may regulate the expression of ETS-۱. In this study, the relation of miR-۱۲۵b on ETS-۱ gene would be investigated.Methods: In this project, the expression levels of miRNAs would be studied using RT-qPCR. Specific primers would be used to amplify the extraction total RNA that would be converted to cDNA. Data analysis would be performed in order to assess the relation of the miR-۱۲۵b expression with breast cancer incidence and metastasis.Results: It has been expected that the expression of miR-۱۲۵b in invasive breast cancer would be reduced and as a result, the expression level of ETS-۱ proto-oncogene could be up-regulated.Conclusion: The possible role of miR-۱۲۵b could be merged with our previous findings on the expression level of ETS-۱ to uncover the role of this miRNA in breast cancer incidence.

Keywords:

Breast cancer , micro RNA , RT-qPCR , miR ۱۲۵b