Evaluation of the effect of thiosemi carbazone complex Ni on expression changes of CASC۲ lncRNA in controlling of Notch signaling pathway in jurkat E.۶.۱

Background and Aim: Acute lymphoblastic leukemia is a malignant bone marrow disease in which the primary lymphoid precursors proliferate and replace the normal blood-forming cells of the brain.ALL is the most common cancer in children.detriment of a group of chemotherapy drugs in prior investigation,such as cis-platin gave rise to an impetus to study the thiosemicarbazone complex. Thiosemicarbazone are effective anticancer agents and act as potent inhibitors of DNA synthesis by inhibiting ribonucleotide reductase. The goal of this study was to evaluate the efficacy of thiosemicarbazones complex on the expression of CASC۲ in a T-all cell line (Jurkat E۶.۱). Methods: First, thiosemicarbazones complex Ni was provided, Next, various doses of Ni were inspected with MTT test. Subsequently, the thiosemicarbazones complex Ni was prepared at ۲ and ۵ µM doses and the jurkat E.۶.۱ Cancer cells were treated with mentioned doses. Next, miRNA extraction and cDNA synthesis were performed and the expression of CASC۲ gene was appraised by Real Time PCR and GAPDH used as a house keeping gene. Finally, the results were analyzed by Rest Software.Results: The results of this research indicated that the expression of CASC۲ significantly increment after treatment with Thiosemicarbazones complex Ni with (p-value < ۰.۰۰۱)Conclusion: in inference, the yield results of the current study lead to the conclusion that doses of ۲ and ۵ µM Ni in ۴۸ hours are the optimal doses and time of the effect of this complex, and the expression of them are ۳.۹۱۸ and ۳.۹۲۳.

Keywords:

CASC۲ , Acute Lymphoblastic Leukemia , Thiosemicarbazones Ni , jurkat E.۶.۱ , GAPDH , Notch signaling