The study of effect of propolis extract on Beta-catenin gene expression in Gastric Adenoma Carsinoma cell line AGS

Background and Aim: Gastric cancer is a multifactorial disease. One of the environmental risk factors involved in the development of gastric cancer is the role of Helicobacter pylori infection. ᵦ-catenin is a dual protein in humans encoded by the CTNNB۱ gene and is involved in the regulation and coordination of cell adhesion and transcription. The aim of this study was to investigate the effect of anti-cancer properties of bee propolis extract on ᵦ-catenin gene expression on AGS cell line.Methods: First, bee propolis was extracted and the materials used in the cell culture process were aliquoted. Then, trypsinization of AGS cells with bee propolis extract at concentrations (۸۰۰ and ۱۲۰۰ µg / ml) in ۴۸ hours, RNA extraction, Cdna synthesis and finally Real Time PCR and statistical data analysis were performed, respectively.Results: The studies performed in this study showed that the ᵦ-catenin gene had a significant decrease in concentrations (۸۰۰ and ۱۲۰۰ µg / ml) over a period of ۴۸ hours, with the highest decrease being related to the concentration (۸۰۰ µg / ml). It was statistically significant.Conclusion: According to these results, ᵦ-catenin gene under a period of ۴۸ hours under the influence of bee propolis extract showed a significant decrease and affected the progression of AGS cells to cell death and these results can be used by knowledge-based companies to produce The drug with bee propolis extract in appropriate concentrations to improve and control the progression of the disease in patients with gastric cancer.

Keywords:

ᵦ-catenin , AGS cells , bee propolis