Identification of Host Specific Protein Pattern in Mycoplasma gallisepticum pMGA۱.۲ protein

Background and Aim: The most abundant membrane proteins in M. gallisepticum are pMGA, lipoproteins of about ۶۷ kDa. Nine members of the pMGA gene M. gallisepticum possesses a large family of pMGA genes encoding variant copies of pMGA hemagglutinins. The pMGA gene family genes has an extraordinary potential for diversifying antigenic structure on the surface of M. gallisepticum cells .The aim of this study was to compare the pMGA protein patterns between different strains and hosts of Mycoplasma gallisepticum , applicable for designing specific recombinant vaccine.Methods: All Mycoplasma gallisepticum full genomes available in Genbank till January ۲۰۲۰ were consider and pMGA۱.۲ sequences were identified , grouped and coded. The nucleotide structure and protein structure were investigated. Deletion, insertion, amino acid substitutions in different strains and host groups were evaluated. Multiple sequence alignment of pMGA۱.۲ with ۶۵۰ length amino acids between two different hosts (galus galus and house finch) were studied by bioinformatics software in all Mycoplasma gallisepticum full genomes.Results: Thirteen host specific amino acids A۱۸۴ ? S , R۴۵۲ ? K , S۴۷۷ ? N , M۵۰۹ ? K , K۵۱۲ ? M , V۵۳۰ ? I , G۵۵۰ ? R , D۵۶۱ ? H , N or T۶۰۱ ? D , N۶۰۲ ? deletion , V۶۰۹ ? L , N۶۲۹ ? S , T۶۴۰ ? S were identified in two host groups galus galus and house finch respectively. Two amino acid patterns were observed between poultry and house finch. Four host related epitopes were identified.Conclusion: Due to the difference in the amino acids and epitope patterns between two hosts, it is recommended that two types of recombinant vaccines might be designed based on the pMGA۱.۲ structure for galus galus and house finch.

Keywords:

pMGA , Mycoplasma gallisepticum , surface protein