The significant change in Antibiotic-resistant gene expression of Acinetobacter baumannii by using functionalized carbon nanotubes Nanofluid

Background and Aim: Acinetobacter Baumannii is one of the most critical factors in the development of nosocomial infections around the world and carries the Tet (A) gene encoding a tetracycline efflux pump. Recently, Carbon nanotubes have described as a novel and potential antibacterial and Nanocarrier agents. Aim: In this research, Antimicrobial effects study of carbon nanotubes Nanofluid on Acinetobacter baumannii and gene expression study on the Tet (A) gene was done.Methods: Cell viability assay was carried out after incubation of Acinetobacter baumannii by the CNT's suspensions (۱۰۰ µg /ml) and antibiotic for ۲۴, ۴۸, and ۷۲ h. Then the gene expression profile of the Tet (A) gene was studied by Real-Time PCR method on bacteria, which were treated by functionalized carbon nanotubes Nano fluid+ Antibiotic in comparison with Antibiotic alone.Results: Results were shown significantly, reduced bacterial proliferation, and reduced expression of the Tet (A) gene in the Nano fluid+ Antibiotic condition. Conclusion: The present study showed that the functionalized carbon nanotubes Nanofluid could be a suitable drug delivery vehicle for overcoming bacterial Antibiotic-resistant.

Keywords:

Antibiotic-resistant , Acinetobacter baumannii , Carbon nanotubes , Nanofluid , Tet (A) gene.