Simultaneous effect of CoCl۲-induced hypoxic condition and Thymoquinone treatment on the expression of Sox۲ gene in the cancerous MCF-۷ cell line

Publish Year: 1399
نوع سند: مقاله کنفرانسی
زبان: English
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CIGS16_351

تاریخ نمایه سازی: 14 اردیبهشت 1400

Abstract:

Background and Aim: Thymoquinone (TQ), as a major compound of Nigella Sativa seeds, has been reported to possess anti-tumor activity against variety of tumors such as breast cancer. Cobalt II chloride (CoCl۲) is a well-known hypoxia mimetic mediator which induced hypoxia-like responses through the induction of hypoxia-inducible factor-۱ (HIF-۱). The present study conducted to investigate the simultaneous effect of CoCl۲-induced hypoxic condition and TQ treatment on the expression of Sox۲ gene in the cancerous MCF-۷ cell line.Methods: MCF-۷ cells were cultured in T۲۵ flasks in DMEM-F۱۲ medium supplemented with ۱۰% fetal bovine serum. The cultured cells at ۷۰% confluency were treated with CoCl۲ (۱۰۰ μmol/L) as control group and with CoCl۲ and TQ (۵۰۰ ng/ml) as treatment group for ۲۴ h. RNA extraction, DNase I treatment and cDNA synthesis were then performed in control and treatment groups. Finally, quantitative real-time PCR (qRT-PCR) was used to evaluate the expression of Sox۲ gene in treated samples compared to the untreated cells.Results: In this study, the effect of TQ on the mRNA expression of Sox۲ gene involved in the proliferation of MCF-۷ cancerous cell line were evaluated under CoCl۲-induced hypoxic condition. The results of qRT-PCR indicated that TQ at CoCl۲-induced hypoxic condition decreased the expression of Sox۲ gene compared to normoxic condition.Conclusion: SOX۲ protein regulates multiple malignant processes of breast cancer such as promotes cell proliferation and thus tumorigenesis through facilitating the G۱/S transition. According to the results of this study, we conclude that TQ can inhibit MCF-۷ cell line proliferation under hypoxic condition. This study provides a good perspective for further efforts to investigate the effect of TQ on different cancerous cell lines and tumors under hypoxic condition for cancer treatment applications.

Authors

Nakisa Ghamari

Biology Department, Razi University, Kermanshah, Iran

Sajjad Sisakhtnezhad

Biology Department, Razi University, Kermanshah, Iran