Analysis of Interleukin-۱۰ Gene Polymorphisms in Patients with Idiopathic Chronic Urticarial in East-Azerbaijan

Background and Aim: Idiopathic chronic urticaria (ICU), is the most common form of chronic urticaria. Because the hives are multifactorial diseases, several mechanisms have been proposed to explain the cause of the disease, especially genetic polymorphism plays an important role in inflammatory disorders. The aim of this study was to evaluate polymorphism (rs۱۸۰۰۸۹۶ - ۱۰۸۷) IL - ۱۰ in relation to the chronic urticarial Methods: A total of ۱۰۰ patients with chronic urticaria and ۱۰۰ control were studied. DNA was extracted from the plasma of patients by DNA extraction kit (Sinaclon-Iran). Polymorphism (rs۱۸۰۰۸۹۶ - ۱۰۸۷) IL -۱۰ was investigated by RFLP PCR.Results: Out of ۱۰۰ patients with chronic urticaria, ۲۹ (۲۹%), ۵۲ (۵۲%), and ۱۹ (۱۹%) sample had GG, AG, and AA, respectively. In the control group, ۲۹ (۲۹%), ۲۲ (۲۲%) and ۴۹ (۴۹%) had GG, AG and AA, respectively. Data analysis showed a significant relation (P =۰.۰۰۹) between the polymorphism (rs۱۸۰۰۸۹۶ - ۱۰۸۷) with the chronic urticaria. AA genotype had a significant relation with chronic urticaria disease (P =۰.۰۰۴ and OR =۴.۰۵۲). Between AG genotype and chronic urticaria (P = ۰.۰۰۳ and OR = ۰.۵۴۷), there was a significant relationship but in the opposite. There was no relation with GG and disease (OR = ۰.۶۹۹; P = ۰.۵۴۱).Conclusion: The results confirm other studies of polymorphisms in genes involved in chronic inflammatory diseases. These results show the different roles of genes among different ethnic groups and different polymorphisms that can explain various clinical protests.

Keywords:

Hives , Chronic urticaria , IL-۱۰ , Polymorphism