Down-regulation of Survivin involved in copper (II) phenylthiosemicarbazone complex-induced apoptosis in leukemia stem-like KG۱a cells

Background and Aim: Previous studies suggested that phenylthiosemicarbazones considered as a new apoptosis-inducing agent. In this study, anti-proliferative and apoptotic effects of the copper (II) phenylthiosemicarbazone complex (Cu-PTSC) were investigated in human acute myeloid leukemia KG۱a cell line. The KG۱a cells were treated with various concentrations of the Cu-PTSC (۲۰-۱۴۰ μM), and cell viability was determined by MTT assay.Western blotting showed that survivin expression considerably down-regulated ۲۴-۷۲ h after treatment with an IC۵۰ concentration of the Cu-PTSC. the cytotoxicity of an active compound from copper (II) phenylthiosemicarbazones (Cu-PTSC) was investigated against KG۱a cells a model for leukemic stem cell in AML. We were interested in understanding its cytotoxic effect and underlying mechanisms of action of the compound on KG۱a cells. This compound caused a significant decrease in the KG۱a cell viability in a dose and time-dependent manner.Methods: Cell culture Western blotting analysis Results: Cu-PTSC induced apoptosis in KG۱a cells Cu-PTSC inhibited KG۱a cell viability Conclusion: Based on present data, it seems that Cu-PTSC may provide a novel therapeutic approach for the treatment of acute myeloid leukemia

Keywords:

Apoptosis , Cu-PTSC , Survivin , KG۱a cell line