Pegah Mousavi - Department of Medical Genetics, Faculty of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas,Iran
Mohammad Hossein Morowvat - Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, P.O. Box ۷۱۴۶۸-۶۴۶۸۵, Shiraz,Iran
Khadijeh Ahmadi - Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Omid Sheydaei - Department of Chemistry, Faculty of Sciences, Yasouj University, Yasouj, Iran

Background and Aim: Prerequisite achieving better life quality is identification of the daises with sensitive, rapid and continuous methods. Sensors and biosensors have many applications in clinical diagnosis, pharmacogenetic researches, drug discovery, gene analysis, identification of viruses and bacteria, and detection of bioterrorism and biological warfare agents. In fabrication of these devices, the use of nanomaterials causes increment in the sensitivity and possibility to use in more complex matrices. In the study, a genosensor (microRNA-based biosensor) fabricated by gold nanoparticles for a rapid, simple and low-cost detection of Brest cancer.Methods: Gold nanostructures were synthesized using sonoelectrodeposition method. SEM and voltammetric methods were employed to characterize the nanostructures. a specific probe was firstly immobilized on the gold nanoparticles. Then, hybridization process with non-complementar and complementary sequences were investigated by electrochemical methods.Results: The figure of merit of the Breast cancer genosensor revealed that this genosensor had an ultrasensitivity (LOD from the order of zeptomolar) and ability to detection the complementary sequence without PCR.Conclusion: So, suggested biosensor enables miR-۱۲۲ detection at very low concentrations and may be an extended measurement strategy easily expanded to identify other miRNAs and is a promising tool for basic research and clinical application.

Genosensor; Gold nanoparticles, Breast cancer