motA gene effects on expression of MALAT۱, GAS۵, NEAT۱, and PVT۱ lncRNAs in Hep G۲ cell line

Background and Aim: Cancer treatment has become a major topic in recent decade. This study purpose was to investigate novel therapeutic methods for cancer. Bacteria has been the topic of many researches due to their anticancer effects. Some genes of Salmonella Typhimurium (S. Typhimurium) for instance motA gene are known to play anticancer role. On the other hand, the expression of some long non-coding RNAs (lncRNAs) such as GAS۵, MALAT۱, NEAT۱ and PVT۱ is regularly apperceived in some types of cancer.Methods: In this study, the differential expression of lncRNAs and mRNAs in hepatocellular carcinoma progression were explored by analyzing of cibioportal. PPI and co-expression networks were created to show the roles of lncRNAs in hepatocellular carcinoma. Also, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were performed to identify functions of differentially expressed genes in hepatocellular carcinoma. Then, the effect of S. Typhimurium motA gene on induction of apoptosis pathways on Hep G۲ cell line was investigated.Results: After creation of lncRNA-diseases interaction, changes in expression levels of GAS۵ (P = ۰.۰۰۲۴), MALAT۱ (P = ۰.۰۰۲۷), NEAT۱ (P = p= ۰.۰۰۳۳) and PVT۱ (P = ۰.۰۰۹۲) were identified, as potential apoptosis biomarkers for hepatocellular carcinoma, after treating Hep G۲ cell line by pCDNA۳.۱ (+)-motA compared to the empty vector. In addition, the flow cytometry data showed transfection by pcDNA۳.۱ (+)-motA could increase Hep G۲ cell apoptosis level (P< ۰.۰۰۰۱) compared to the pcDNA۳.۱ (+) group.Conclusion: This study findings suggested that S. Typhimurium motA gene could provide a new landscape for researches aimed to increase apoptosis in the transfected cancer cell lines.

Keywords:

Long Non-Coding RNA , Apoptosis , motA gene , hepatocellular carcinoma