MiR-۱۴۳ inhibits migration of SW-۴۸۰ colorectal cancer cells via downregulation of MMP-۹ and ROCK

Background and Aim: Colorectal cancer (CRC) is considered as the third common cause of cancer-related mortality worldwide. Treatment failure is the major reason for the low survival rates of CRC patients. Therefore, the identification of molecular mechanisms underlying CRC tumorigenesis is essential for the improvement of therapeutic strategies. MicroRNAs (miRNAs), a class of regulatory small non-coding RNAs are dysregulated in human cancers and involved in its development.MiR‑۱۴۳, as a tumor suppressor gene, was also shown to play essential roles in cancer progression including migration. Given that, in this study, we investigated the effect of miR-۱۴۳ replacement on the migration of SW-۴۸۰ human colorectal cancer cells.Methods: SW-۴۸۰ cells purchased from National Cell Bank of Iran were cultivated in complete RPMI-۱۶۴۰ medium. As cell monolayers reached ۷۰-۸۰% confluence, they were transfected with miR-۱۴۳ mimics using electroporation. Subsequently, wound healing assay was used to measure the migratory ability of transfected cells compared to the control. Moreover, using quantitative real-time PCR (qRT-PCR), the expression levels of miR-۱۴۳, MMP-۹, and ROCK were investigated in treatment groups.Results: The obtained results showed that miR-۱۴۳ expression levels were upregulated after transfection in SW-۴۸۰ cells. Wound healing assay illustrated that miR-۱۴۳ overexpression significantly reduced HT-۲۹ cell migration. Furthermore, qRT-PCR results also demonstrated that miR-۱۴۳ replacement downregulated MMP-۹ and ROCK mRNA expression levels.Conclusion: Taken together, our results implied that miR-۱۴۳ could inhibit SW-۴۸۰ cell migration by modulating the expression of metastasis-related genes, confirming the therapeutic value of this miRNA in CRC.

Keywords:

Colorectal Cancer , miR-۱۴۳ , Metastasis