Evaluation of hsa-miR-۷۶۶-۵p as a new regulator of mitochondrial apoptosis pathway and cardiomyocyte differentiation of Human embryonic stem cells (hESCs)

Background and Aim: miRNAs are small non coding mediator RNAs with potential role in development, cellular proliferation, differentiation, and apoptosis. These agnets can be used as new regulator of mitochondrial apoptosis pathway. The aim of this study is examination of the role of hsa-miR-۷۶۶-۵p during the apoptosis and cardiomyocyte differentiation of Human embryonic stem cells (hESCs).Results: Flow cytometry and MTT assay indicated cell death reduction and viability elevation effect of hsa-miR-۷۶۶ in SW۴۸۰ cells after its overexpression. Endogenous expression of hsa-miR-۷۶۶ during the course of human embryonic stem cells (hESCs) differentiation into cardiomyocytes revealed an inverse expression status of this miRNA with BOK. However, the expression of this miRNA was inversely related to BAX and BAK for some time points of differentiation.Conclusion: Based on this study, the involvement of hsa-miR-۷۶۶ in regulation of mitochondrial apoptosis pathway was approved.

Keywords:

Apoptosis , differentiation , cell death , miRNA , cardiac cell , miR-۷۶۶