ناشر تخصصی کنفرانس های ایران

لطفا کمی صبر نمایید

Publisher of Iranian Journals and Conference Proceedings

Please waite ..
Publisher of Iranian Journals and Conference Proceedings
Login |Register |Help |عضویت کتابخانه ها
Paper
Title

Investigation of therapeutic effects of stem cell (hAMSC) on pancreatic cancer cell (MIA PaCa۲) by evaluating of epidermal growth factor receptor (EGFR) expression level

Year: 1399
COI: CIGS16_453
Language: EnglishView: 202
نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد

Authors

Zahra Rahmani - Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran.
Fatemeh Safari - Department of Biology, Faculty of Science, University of Guilan, Rasht, Iran.

Abstract:

Background and Aim: Mesenchymal stem cells (MSCs) are a population of pluripotent stem cells with potential applications in regenerative medicine and tumor targeting therapy. It was found that tumors are under the effects of tumor microenvironment, which consisted of mesenchymal stem cells (MSCs), growth factors, cytokine networks, tumor vasculature, and extracellular matrix. The interactions between MSCs and tumor cells are complicated, and the mechanisms involved are multifaceted. The study of molecular events associated with MSC/tumor cell interactions would be essential for identifying the role of these important stem cells in physiological and pathophysiological conditions. In this study, we investigated the possible effects of hAMSC on the pancreatic cancer cell (MIA PaCa۲) by using co-culture method. In this regards, we are interested to evaluate the expression level of EGFR as one of the most critical molecules to regulate the cell signal transduction pathways.Methods: Mesenchymal stem cell from human cord blood (hAMSCs) and pancreatic cancer cell (MIA PaCa۲) were prepared and were separately cultured in Dulbecco's modified Eagle's medium (DMEM) medium with ۱۰% fetal bovine serum and in a sterile humidified incubator with ۵% CO۲ at ۳۷˚C. Then, these two cell lines were co-cultured in double culture plates for ۲۴ and ۷۲ h. MIA PaCa۲ cells were considered the control group and underwent the same culturing time. The RNA of treated and untreated cancer cells was extracted and then EGFR expression was analyzed by using quantitative real time PCR (qRT-PCR). For further study, total cell lysates of related cells were prepared and the expression of EGFR protein was analyzed by using western blot.Results: The expression of EGFR in pancreatic cancer cell under the co-culture with hAMSC was decreased. Of note, the results from western blot analysis confirmed qRT-PCR results.Conclusion: Our results showed that interaction between stem cells with pancreatic cancer cells has inhibitory effects on cancer cells and it can be consider as a therapeutic way to suppress EGFR as a potent target of cancer cells signaling. However, more study will be required to find all critical players in this field.

Keywords:

Stem cells, Cancer cells, EGFR, Co-culture

Paper COI Code

This Paper COI Code is CIGS16_453. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/1195719/

How to Cite to This Paper:

If you want to refer to this Paper in your research work, you can simply use the following phrase in the resources section:
Rahmani, Zahra and Safari, Fatemeh,1399,Investigation of therapeutic effects of stem cell (hAMSC) on pancreatic cancer cell (MIA PaCa۲) by evaluating of epidermal growth factor receptor (EGFR) expression level,the fourth International and 16th National Genetics Congress,Tehran,https://civilica.com/doc/1195719

Research Info Management

Certificate | Report | من نویسنده این مقاله هستم
این Paper در بخشهای موضوعی زیر دسته بندی شده است:

اطلاعات استنادی این Paper را به نرم افزارهای مدیریت اطلاعات علمی و استنادی ارسال نمایید و در تحقیقات خود از آن استفاده نمایید.

Scientometrics

The specifications of the publisher center of this Paper are as follows:
Type of center: دانشگاه دولتی
Paper count: 13,225
In the scientometrics section of CIVILICA, you can see the scientific ranking of the Iranian academic and research centers based on the statistics of indexed articles.

New Papers

Share this page

More information about COI

COI stands for "CIVILICA Object Identifier". COI is the unique code assigned to articles of Iranian conferences and journals when indexing on the CIVILICA citation database.

The COI is the national code of documents indexed in CIVILICA and is a unique and permanent code. it can always be cited and tracked and assumed as registration confirmation ID.

Support