Differentiation of human adipose derived mesenchymal stem cells cultured onto compound semi-conductive Nano-fibrous scaffold into cardiomyocyte cells

Background and Aim: Although cardiac mortality has decreased in the past few years, cardiac diseases are still responsible for millions of death every year worldwide. The use of mesenchymal stem cells (MSCs) in research and regenerative medicine has improved recently. Human adipose-derived stem cells (ASCs) are good candidate for use in stem cell-based clinical therapies. They could be cultured and differentiated into several lineages such as cartilage, bone, muscle, neuronal cells, etc. Tissue engineering with a combination of stem cells and nanofibrous scaffolds has attracted interest with regard to Cardiomyocyte creation applications.Methods: In the present study, hMSCs were isolated from human adipose tissue and cultured in vitro. The phenotypes of hMSCs were identified by flow cytometric analyses, then they were cultured on polycaprolactone co polyaniline (PCL/Pani) nanofibers. The capacity of PCL/Pani Nanofibrous scaffolds to support stem cells for the proliferation was studied by MTT assay. The differentiation of hMSCs into cardiomyocyte is confirmed with evaluation of specific cardiac related gene markers expression by real-time RT-PCR.Results: The typical morphological features of hMSCs were fibroblast-like in shape. hMSCs expressed CD۴۴ and CD۱۰۵, but did not express CD۳۴ and CD۴۵. The differentiation rate of ASCs on PCL/Pani was significantly high. The expression rate of cardiovascular related gene markers such as GATA۴ and MYH۷ was significantly increased in PCL/Pani nanofibers.Conclusion: The results indicated that the compound PCL/pani nanofibers are suitable physical properties as polymeric artificial scaffold in cardiovascular tissue engineering application.

Keywords:

Adipose-derived stem cells , Nanofibrous scaffolds , cardiomyocytes , cell therapy , cell differentiation