Z. Piervandi - Mineral Processing Group, Department of Mining Engineering, Tarbiat Modarres University, Tehran, Iran
A. Khodadadi Darban - Mineral Processing Group, Department of Mining Engineering, Tarbiat Modarres University, Tehran, Iran
Seyed M. Mousavi - Biotechnology Group, Department of Chemical Engineering, Tarbiat Modarres University, Tehran, Iran
M. Abdollahi - Mineral Processing Group, Department of Mining Engineering, Tarbiat Modarres University, Tehran, Iran
Gh.R. Asadollahfardi - Faculty of Engineering, Department of Civil Engineering, Kharazmi University, Tehran, Iran
K. Akbari Noghabi - National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

Indigenous acidophilic bacteria separated from mine-waste can be used in return for the addition of the reagents like sulfuric acid. Among the tailings bacteria, Acidithiobacillus ferrooxidans and Acidithiobacillus thiooxidans are of the most-studied ones for the bioleaching and bioremediation of elements. In this work, the isolation and characterization of the mentioned bacteria are studied by a proposed biochemical protocol. The sequential cultivation of the soil bacteria in a series of liquid media and solid cult