H Jafarzadeh - Department of Animal Science, AharFaculty of Agriculture and Natural Resources, University of Tabriz, Ahar, Iran;
M Allymehr - Department of Obstetrics and Poultry, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
A Talebi - Department of Obstetrics and Poultry, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
S Asri - Department of Internal Diseases and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
A Soleimanzadeh - Department of Obstetrics and Poultry, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Objectives:Selenium is a trace element that is discovered and admitted for nearly ۵ decades. It actively participates in oxidative stress resistance and reproductive performance. For male reproductive system development, optimum level of Selenium in testis is required. The aim of this study was to conduct a survey about effects of different forms of dietary selenium (Se) supplementation, mainly Nano-Se on gene expression of Selenoprotein P (SelP), Glutathione peroxidase ۴ (GPx۴) and Selenoprotein W (SelW) in broiler breeder rooster’s testis. Materials & Methods:A total of ۳۰ Arbor Acres Broiler Breeder males (more than ۴۰ weeks) were randomly divided into five groups, each of which included ۳ replicates of ۲ birds. These five groups contained a control group and four treatments receiving the same basal diet supplemented with ۰.۳ mg Se/kg as Sodium Selenite (SS) in T۱ or ۰.۱۵ mg Se/kg as Nano-Selenium in T۲ or ۰.۳ mg Se/kg as Nano-Selenium in T۳ or ۰.۶ mg Se/kg as Nano-Selenium in T۴ for ۴ weeks after one week of adaptation. After experimental period, testis samples were collected for detecting expression of three specific SelP, GPx۴ and SelW genes. Results & Conclusion:The results indicated that byincreasing Nano-Se level in the diet, the mRNA expression level was increased and the highest mRNA expression of all three mentioned genes was observed in the treatment of ۰.۶ mg/kg Nano- Selenium (T۴). Also, at the same amount of different types of Se sources (Nano-Se vs Sodium Selenite (SS)) the Nano-Se compared to the inorganic form (SS) had greater mRNA expression of selenoprotein genes.

Broiler Breeder, GPx۴, Nano-Selenium, SelP, SelW.