asad kazemi - Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
faranak elmi - Biology Dept., Univesity of Tabrize, Tabrize, I.R.Iran
alireza moradi - Department of Physiology, Medical School, Ardabil University of Medical Sciences, Ardabil, Iran

Backgruon and aims: The aim of this research was to compare the chemical composition and the antioxidant and antibacterial properties of wild and cultivated types of T. fedtschenkoi collected from East Azarbayjan, Iran. Material and Methods: The essential oils (EOs) from aerial parts of wild and cultivated T. fedtschenkoi were investigated by gas chromatography/mass spectrometry (GC/MS). Antibacterial activities of EOs and methanol extracts were tested against bacteria by disc diffusion method and determining their minimum inhibitory concentration (MIC) values by agar dilution method and antioxidant activity by DPPH and FRAP assays. Results: Twenty-two components were identified representing more than ۹۹.۲۰% of the total oil constituents. The major components in the oils of the wild type were; thymol (۴۹.۲۱%), p-cymene (۱۵.۲۰%) and Carvacrol (۱۰.۲۰%) and for the cultivated type were; thymol (۶۲.۳۳%) and carvacrol (۷.۱۲%). The MIC values of bacterial strains, which were sensitive to the EO of T. fedtschenkoi, were in the range of ۲-۱۲۸ μg/mL in wild type and ۲-۱۶ μg/mL in cultivated type. Conclusion: Our data show that cultivation significantly affects the EOs’ chemical composition and antioxidant potential of T. fedtschenkoi. They signify a reasonable source of natural antibacterial substances that proved to be potential as a drug for use in pathogenic bacteria.The antibacterial activity by the presence of inhibition zone appeared from EOs in the cultivated type on all tested microorganisms significantly higher than that in the wild type, which can be attributed to the presence of high concentration of thymol (۴۷.۴۸%) against the wild type (۲۹.۹۶%).

Biological activity, cultivation, Essential oil composition, Thymus fedtschenkoi