Khadijeh Rezaie.Keikhaie - Department of Obstetrics and Fetal Health Research Center, Zabol University of Medical sciences, Zabol, Iran.
Gholamreza Bagheri - Zabol Medicinal plant Research Center, Zabol University of Medical Sciences, Zabol, Iran
Saeide Saeidi - Center of Agricultural Biotechnology, University of Zabol, Zabol, Iran
Mehdi Hassanshahian - Department of Biology- Faculty of Scienc- Shahid Bahonar University of Kerman- Kerman- Iran.

Background and aims: The purpose of this study was to evaluate the antimicrobial effects of zinc oxide nanoparticale and extract of Solanum nigrum on Pseudomonas aeruginosa bacteria isolated from clinical specimens.Methods: Zinc oxide was purchased from the market. ۲۰g of the dried and pounded leaves of S. nigrum was used and its extract was prepared in rotary device. ۱۲ isolates of P. aeruginosa were isolated from referred patients to hospital Zabol. Finally, ۱۲ isolates were used to growth inhibitory activity assay. Minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) of Zinc oxide and extract plant against P.aeruginosa were evaluated using micro broth dilution method.Results:The highest inhibitory concentration for P. aeruginosa is ۱۵۰۰ μg/ml, with four bacterial strains being inoculated. Also, the results showed that the highest drainage concentration was ۳۰۰۰ μg/ml, which two strains were inhibited in this concentration and the lowest trap concentration was ۹۳ μg/ml. The lowest inhibitory concentration of extract plant was ۰.۶۲ mg / ml, with only one strain being inhibited in this concentration. The highest inhibitory concentration for P. aeruginosa is ۴۰ mg / ml, with four bacterial strains being inoculated.Conclusion:The results showed that zinc oxide and extract of Solanum nigrum have a good antimicrobial activity on the bacterium and increases the concentration of antimicrobial activity.

Zinc oxide nanoparticles, Solanum nigrum, Antimicrobial activity, Pseudomonas aeruginosa