Amir Hossein Mansourabadi - ۱Immunology Dept., Shahid Sadoughi University of Medical Sciences, Yazd, I.R. Iran
Hamid Mohammad Sadeghi - ۲Physiology Dept, Shahid Sadoughi University of Medical Sciences, Yazd, I.R. Iran
Nastaran Razavi - ۳Shahrekord University of Medical Sciences, Shahrekord, I.R. Iran
Ebrahim Rezvani - ۲Physiology Dept, Shahid Sadoughi University of Medical Sciences, Yazd, I.R. Iran

Background and aims: Inflammation is one of the defense mechanisms of body and unpleasant sensation of pain is caused by tissue damage. Mostly, inflammation occurs through the release of inflammatory mediators. Salvia officinalis is one of the most valuable medicinal kind of mint order. Salvigenin is one of the active flavonoids existing in this plant. The aim of this study was to evaluate the anti-inflammatory and analgesic effect of salvigenin, Salvia officinalis flavonoid extracted. Methods: In this laboratory experimental study, plant was extracted and the column chromatography was used to purify prepared extracts. ۱۰۰ male albino mice and ۴۸ male wistar rats were selected. In the hot plate test and in the writhing test, animals were divided randomly into ۵ groups. Group ۱ (received ۱۰ mg/kg normal saline), groups ۲, ۳ and ۴ (received Salvigenin ۲۵, ۵۰ and ۱۰۰ mg/kg intraperitoneally, espectively), group ۵ (received ۱۰ mg/kg morphine in hot plate test and ۱۰ mg/kg indomethacin in writhing test). In the inflammatory test, animals were divided into ۶ groups. Group ۱ was assigned as a control group which received ۰.۰۵ ml of carrageenin. Groups ۲, ۳ and ۴ (received Salvigenin, at doses of ۲۵, ۵۰ and ۱۰۰ mg/kg). Group ۵ (received ۱۰ mg/kg indomethacin) and then changes of the volume of all groups were measured. Data were analyzed using ANOVA and Tukey test and PResults: In writhing test, Salvigenin reduced the number of abdominal contractions at doses of ۵۰ and ۱۰۰ mg/kg. Increasing dose of Salvigenin, with reduction in abdominal cramps resulted in the increasing of pain inhibition, and the percentage of this inhibition was statistically significant (P<۰.۰۰۱). In hot plate test, also ۳۰, ۴۵ and ۶۰ minutes after injection of Salvigenin and morphine showed significant difference compared to the control group (P<۰.۰۰۱). Also, Salvigenin increased the maximum percentage of analgesic compared to the control group (P<۰.۰۰۱). Salvigenin could reduce inflammation and in the group that received Salvigenin at ۱۰۰ mg/kg, the inflammation was significantly lower than the control group (P<۰.۰۵). Discussion: Our findings showed that Salvigenin has dose-dependent analgesic effect so that it can be useful in controlling of inflammations, acute and chronic pain.  

Salvigenin, Flavonoid, Salvia officinalis, Inflammation, Pain