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Title

Downregulation of Stemness Genes and Induction of Necrosis in Rat LA۷ Cancer Stem Cells Induced Tumors Treated with Starved Fibroblasts Culture Supernatant

Year: 1400
COI: JR_RBMB-10-1_012
Language: EnglishView: 125
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Authors

Roghayeh Pourbagher - Student Research Committee, Babol University of Medical Sciences, Babol, Iran & Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Hossein Ghorbani - Department of Pathology, Rohani Hospital, Babol University of Medical Sciences, Babol, Iran.
Haleh Akhavan-Niaki - Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Seyed Gholam Ali Jorsaraei - Fatemeh Zahra Infertility and Reproductive Health Research Centre, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Sadegh Fattahi - Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Sahar Ghooran - Department of Pathology, Rohani Hospital, Babol University of Medical Sciences, Babol, Iran.
Zeinab Abedian - Student Research Committee, Babol University of Medical Sciences, Babol, Iran & Dental Materials Research Center, Dental Faculty, Babol University of Medical Sciences, Babol, Iran.
Masoumeh Ghasemi - Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Fatemeh Saeedi - Student Research Committee, Babol University of Medical Sciences, Babol, Iran & Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Negar Jafari - Student Research Committee, Babol University of Medical Sciences, Babol, Iran & Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.
Behnam Kalali - Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München, Munich, Germany.
Amrollah Mostafazadeh - Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran.

Abstract:

Background: Stem cell differentiation therapy is a promising strategy in cancer treatment. we show that protein cocktail prepared from serum starved fibroblasts has therapeutic potential based on this strategy. Methods: The condition medium was prepared from foreskin isolated fibroblasts and analyzed by Liquid chromatography electrospray ionization mass spectrometry-mass spectrometry (LC-ESI-MS/MS). LA۷ mammary gland cancer stem cells originated tumors were induced in Sprague Dawley rats. The rats treated subcutaneously with DMEM (group A), condition medium (group B), or normal saline (group C) once daily for ۷ days. Then the tumors were removed and divided into the two parts, one part was used to quantify gene expression by stem-loop RT-qPCR assay and the other part was used for Hematoxylin & Eosin (H & E), Giemsa, and immunohistochemistry (IHC) staining. Results: All induced tumors appeared as sarcomatoid carcinoma (SC). Immunohistochemistry staining confirmed this conclusion by recognizing the tumor as Ki۶۷+, cytokeratin+, vimentine+, and estrogen receptor negative SC. RT-qPCR analysis revealed that Oct۴-, Sox-۲, Nanog- gene expression was much reduced in the condition medium treated tumors versus proper controls (p< ۰.۰۵). Tissue necrosis was more prevalent in this group while tumors volume was diminished almost by ۴۰%. The LC-ESI-MS/MS analysis unrevealed the stemness reducing and the cell death inducing proteins such as, pigment epithelium-derived factor (PEDF), insulin like growth factor binding protein-۵ (IGFBP-۵) and -۷ (IGFBP-۷) in the condition medium. Conclusions: This study showed that the substances released from starved human fibroblasts were able to down-regulate the stemness-related genes and induce necrosis in LA۷ derived tumors.

Keywords:

Paper COI Code

This Paper COI Code is JR_RBMB-10-1_012. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/1225396/

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If you want to refer to this Paper in your research work, you can simply use the following phrase in the resources section:
Pourbagher, Roghayeh and Ghorbani, Hossein and Akhavan-Niaki, Haleh and Jorsaraei, Seyed Gholam Ali and Fattahi, Sadegh and Ghooran, Sahar and Abedian, Zeinab and Ghasemi, Masoumeh and Saeedi, Fatemeh and Jafari, Negar and Kalali, Behnam and Mostafazadeh, Amrollah,1400,Downregulation of Stemness Genes and Induction of Necrosis in Rat LA۷ Cancer Stem Cells Induced Tumors Treated with Starved Fibroblasts Culture Supernatant,https://civilica.com/doc/1225396

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