Synergic Effect of Curcuma longa L. Extract with Antimicrobials against Cuban Helicobacter pylori Isolates

Aims: Helicobacter pylori is a bacterium that colonizes the gastric mucosa and is the main cause of gastritis as well as ulcer and gastric cancer. Due to the clinical significance and international increase in H. pylori multidrug resistance, it is necessary to search for new strategies improving eradication rates. Natural compounds have been demonstrated to have antimicrobial effect and the ability to restore the efficacy of conventional drugs. The objective of this work was to evaluate the antimicrobial effect of the hydroalcoholic extract of Curcuma longa L. (Cu) against H. pylori isolates. Materials & Methods: The minimum bactericidal concentration of the extract was determined by means of the MTT assay; also, the combination and dose reduction indices for levofloxacin (LVX), metronidazole (MET), and rifampicin (RIF) antimicrobial agents were determined by checkerboard format. Interaction analysis was performed using the CompuSyn program. Findings: About ۹۰% of H. pylori isolates studied (۹/۱۰) were sensitive to the hydroalcoholic extract. Synergism was observed in more than ۵۰% of Cu-LVX, Cu-MET, and Cu-RIF combinations. Additionally, for different concentrations of the extract, reduction rates in antimicrobial agents were determined to be between ۰.۵ and ۳۶۰ times. Conclusions: The hydroalcoholic extract of turmeric showed a good potential to be used as an antimicrobial agent in the treatment of H. pylori infection, either alone or in combination with antibiotics used, suggesting the renewal of the effectiveness of conventional antimicrobials in reducing the phenomenon of antimicrobial resistance.

Keywords:

Helicobacter pylori , Antimicrobial resistance , Curcuma longa , Drugs combination.