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Title

The Optimized Protocols for Production, Adaptation and Keeping of the Produced Artificial Seeds from Encapsulated Lateral Buds in Stevia rebaudiana (Bertoni)

Year: 1400
COI: JR_ATIC-1-1_004
Language: EnglishView: 71
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Authors

Bahare Shaafi - Department of Agronomy and Plant, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
Sayed Saeed Mosavi - Department of Agronomy and Plant, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
Mohamad Reza Abdollahi - Department of Agronomy and Plant, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran
Hasan Sarikhani - Department of Agronomy and Plant, Faculty of Agriculture, Bu-Ali Sina University, Hamedan, Iran

Abstract:

Stevia, as an anti-diabetic medicinal plant, is estimated to be about ۳۰۰ times sweeter than sugar cane. Low seed germination is a major problem in stevia propagation.  Artificial seeds (ASs) technology is a valuable method for it’s rapid and massive propagation. In this study, the effective factors on production, the produced plantlet adaptation and keeping of the ASs produced from encapsulated lateral buds were efficiently optimized for the first time. The results showed that ۲.۵% sodium alginate with ۱% calcium chloride was the best composition for coating the ASs. The bigger lateral buds (۲-۳ mm) with MS medium resulted in the highest percent and rate of germination. Germination and growth of the ASs were increased using ۱ mg l-۱ BAP with ۱.۵ mg l-۱ Kn in the pre-culture environment. The findings indicated that transmission of the ASs-plantlets to cocopeat   seedbed increased the growth characters. Keeping the produced ASs in the MS medium had a significant (p <۰.۰۵) and positive effect on germination characters. Among the treatments for keeping artificial seeds in ۹۰ days, the treatment of liquid paraffin at ۴oC showed the best response for regrowth. In this research, the possibility of routine and massive production of ASs by encapsulating the lateral buds has been reported in stevia. Therefore, using this new protocol, the rapid and fast micro-propagation of this plant could be achieved through lateral buds for inexpensive and commercial purposes in the future.

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Paper COI Code

This Paper COI Code is JR_ATIC-1-1_004. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/1247533/

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Shaafi, Bahare and Mosavi, Sayed Saeed and Abdollahi, Mohamad Reza and Sarikhani, Hasan,1400,The Optimized Protocols for Production, Adaptation and Keeping of the Produced Artificial Seeds from Encapsulated Lateral Buds in Stevia rebaudiana (Bertoni),https://civilica.com/doc/1247533

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  • Reddy M.C., Muthy K.S.R., Pulliaiah T. ۲۰۱۲. Synthetic seeds, Areview ...
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  • https://doi.org/۱۰.۱۰۰۷/s۱۱۷۳۸-۰۰۹-۰۴۴۴-۷Sharma S., Shahzad A. ۲۰۱۲. Encapsulation technology for short- term ...
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