Spectrophotometric Determination of Naproxen Using Chitosan Capped Silver Nanoparticles in Pharmaceutical Formulation

Abstract In this work, determination of naproxen concentration by chitosan capped silver nanoparticles were investigated. A new, simple and inexpensive spectrophotometric method for the first time was developed. The characteristics of chitosan capped silver nanoparticles were determined by ultraviolet spectroscopy (UV), Fourier transform infrared spectroscopy (FT-IR), atomic force microscopy (AFM), dynamic light scattering (DLS) and zeta potential (ZP) under optimal conditions. It worth to be mentioned that, synthesized chitosan (CS) -Ag nanoparticles were approximately ۱۰۰ nm and PDI ۰.۳۸۵ with strong anionic (-۲۴.۸mV) zeta potential in acidic condition. It was found that chitosan as a chiral selector was able to detect naproxen at optimal experimental conditions. Validation of this method, including limit of detection and limit of quantification were accurately confirmed according to ICH instructions. Based on this method, the limit of detection (LOD) and the limit of quantification (LOQ) of naproxen were calculated ۰.۰۲۲ and ۰.۰۶۶ mol. L-۱, respectively. Analysis of statistical data, the reproducibility and accuracy of this method demonstrated that the use of this novel method is valuable and practical for determination of naproxen in pharmaceutical formulations

Keywords:

Chitosan , Naproxen , Silver nanoparticle , UV- vis spectrophotometric determination , Characterization