Simultaneous Effect of Resistance Training and Alpha Lipoic Acid on LC۳-۱ and P۶۲ Gene Expression of Fatty Liver Diabetic Male Rats

Background and Objective: Autophagy is a genetically and evolutionarily conserved the programmed process that destroys long-lived cellular proteins and organelles. This study aimed to evaluate the effect of eight weeks of strength training with alpha-lipoic acid supplementation on LC۳-I and P۶۲ in elderly diabetic rats. Material and Methods: In this experimental study, ۳۵ old male Wistar rats were randomly divided into ۵ groups (healthy control, diabetic, diabetic + strength training, Diabetic + supplement, Diabetic + strength training + supplement). First, the rats became diabetic and had a fatty liver. Strength training program in ۱۰ weeks performed. Alfa-lipoic acid supplement was also injected ۳ days a week. At the end of the training period and tissues were removed and the expression of LC۳-I and P۶۲ were measured by RT-PCR. For data analysis, a one-way analysis variance test was used for comparison between groups was considered (P<۰/۰۵). Results: The result showed mRNA LC۳-۱ has increased and this change is more in the diabetic group. (P=۰.۰۰۱) and  despite the decrease in P۶۲ gene expression, we did not see a significant difference between the control group and the diabetic in the training group with and without supplementation.However, no significant difference was observed between training methods and supplementation with exercise. Also, resistance training in combination with alpha-lipoic acid supplementation had a significant effect on reducing P۶۲ content between control and experimental groups. (P=۰.۰۰۱). Conclusion: Alfa-lipoic acid supplement with anti-oxidant and anti-inflammatory effect along with exercise can play an important role in improving fatty liver in elderly type ۲ diabetic rats.

Keywords:

Exercise Training , Autophagy , Immune System , Dietary Supplements