Abdossaleh Zar - Department of Sport Science, School of Literature and Humanities, Persian Gulf University, Boushehr, Iran/Persian Gulf Sports, Nutrition and Health Research Team, School of Literature and Humanities, Persian Gulf University, Boushehr, Iran
Fatemeh Ahmadi - Persian Gulf Sports, Nutrition and Health Research Team, School of Literature and Humanities, Persian Gulf University, Boushehr, Iran

Background and Objective: Effects of resistance training and herbal supplements on cardiac signaling pathways are sparsely reported in the literature. This study aimed to evaluate the CITED۴ gene expression in the cardiac muscle of male rats as a result of resistance exercise and spirulina supplementation. Material and Methods: Thirty-two rats (male - Sprague Dally) were grouped into ۴ groups (۱. resistance training: RE, ۲. spirulina + resistance training: SP +RE, ۳. spirulina platensis: SP, ۴. control: Co, n = ۸). The training program was performed for healthy training groups ۵ sessions per week for ۸ weeks. Supplementation included ۲۰۰ mg/kg/ day of Spirulina for the supplement groups. Real-time PCR was used to measure gene expression. We used of Two-way ANOVA in SPSS (p<۰.۰۵). Results: In comparison with the control group, we observed a significant increase in CITED۴ gene expression in RE (P= ۰.۰۰۱) and RE+SP (P= ۰.۰۰۱) groups. Also, there was a significant difference in CEBP gene expression between CO with RE (P= ۰.۰۰۱), SP (P= ۰.۰۳۴), RE+SP (P= ۰.۰۰۱) groups. Conclusion: Spirulina supplementation alone has no effect on the signaling pathway of cardiac hypertrophy. However, if used concomitantly with resistance training, it can affect the signal pathway of cardiac hypertrophy.

CITED۴, Hypertrophy, Resistance Training, Spirulina