Optimization of Coenzyme Q۱۰ Production by Gluconobacter japonicus FM۱۰ Using Response Surface Methodology

Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_JABR-8-2_011

تاریخ نمایه سازی: 20 مرداد 1400

Abstract:

Introduction: Coenzyme Q۱۰ is one of the antioxidants with a worldwide market. Nowadays the coenzymeQ۱۰ production has been considered by fermentation using microorganisms. In this study, the Response Surface Methodology was used to optimize culture composition for coenzyme Q۱۰ production by a previously isolated bacterium, Gluconobacter japonicus FM۱۰. Materials and methods: A central composite design was employed to optimize the culture composition including sorbitol, yeast extract, peptone, KH۲PO۴, and MgSO۴ for coenzyme Q۱۰ production. The dry cell weight and coenzymeQ۱۰ concentration were monitored as response variables and the desirability function approach was applied to obtain the optimum level for each factor. Results: Results showed that an average, ۳ mg/L of coenzyme Q۱۰ was obtained when the optimized culture composition was employed (۱۱۰ g/L of sorbitol, ۲۵ g/L of yeast extract, ۳۵ g/L of peptone, ۰.۵ g/L of KH۲PO۴, and ۰.۵۵ g/L of MgSO۴). In addition, the expected dry cell weight reached ۶ g/L in the presence of ۹۰ g/L of sorbitol, ۱۷.۵ g/L of yeast extract, ۳۵ g/L of peptone, ۰ g/L of KH۲PO۴, and ۱.۷ g/L of MgSO۴. Conclusions: The results of regression analysis revealed that the concentrations of peptone and sorbitol were the most effective factors in producing coenzyme Q۱۰ and dry cell weight, respectively. 

Authors

Foozieh Moghadami

Department of Biology, Payame Noor University, Tehran, Iran

Ramin Hosseini

Department of Biotechnology, Faculty of Agriculture and Natural Resources, Imam Khomeini International University, Qazvin, Iran

Jamshid Fooladi

Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran

Mahdi Kalantari

Department of Statistics, Payame Noor University, Tehran, Iran