New Spoligotypes of Mycobacterium tuberculosis isolates in west of Iran

Background and Aim : Spoligotyping can assay the transmission of mycobacterium tuberculosis strains. We aimed to study the geneotyping of M. tuberculosis isolated from patients with tuberculosis from west of Iran by spoligotyping.Methods : a total of ۴۷ M. tuberculosis isolates were collected from west of Iran. All sample cultures on Lowenstein-Jensen medium and incubated at ۳۷ °C for ۸ weeks. Bacterial isolates were identified as M. tuberculosis using standard biochemical tests. Drug resistance patterns of M. tuberculosis were to rifampicin and isoniazid determined and multi drug resistance (MDR) strains isolated. After DNA extraction, Spoligotyping was performed.Results : This study revealed new spoligotypes ۴۱۶۲ and ۴۱۶۳ that correlated to Atypic lineage. Atypic and Unknown lineages also hah correlation with MDR-TB rate (۴%). The most prevalent (Spoligotyping international types) SIT were Orphan (۳۴%), ۲۶۶۹(۲۳.۴%) and ۱۲۷(۱۴.۸%) types. The most prevalent clade were Ural-۲ (NEW-۱) (۲۵.۵۳%) and Atypic (۲۳.۴۰%) lineages.Conclusion : The predominant clade were Ural-۲ (NEW-۱) and Atypic lineages lineage that restricted to Iran. Also the rate of MDR were so low. A good control program need to know the dynamic transmission of local isolates, therefore this study present the circulating isolate in west of Iran.

Keywords:

Iran , Mycobacterium tuberculosis , spoligotyping