Cytotoxicity effect and Changes in the expression of BAX, BCL-۲ and CASPASE-۹ genes in breast cancer cell line (MCF-۷) treated with hydroalcoholic Rosmarinus officinalis extract

Background and Aim : Today, due to the high prevalence of cancer in Iran and the world, the need for drugs with fewer side effects and better therapeutic effects has been considered by researchers. So that more than ۶۰% of anti-cancer compounds for the treatment of cancer patients are obtained from plant sources. The aim of this study was to investigate the effect of hydroalcoholic extract of rosemary (Rosmarinus officinalis) on the expression of BAX, BCL-۲ and CASPASE-۹ genes in breast cancer cell line (MCF-۷).Methods : In this experimental study, the hydroalcoholic extract of rosemary was made qualitatively by Soxhlet apparatus. Cells were treated with doses of ۵,۱۰,۲۵,۵۰ and ۱۰۰ micromole rosemary extract for ۲۴,۴۸ and ۷۲ hours. Then, using MTT method, the effect of different concentrations of the extract on cell life was investigated. Using Real Time PCR, the expression of BAX, BCL-۲ and CASPASE-۹ genes in the groups treated with different amounts of rosemary extract was measured.Results : This study showed that with increasing concentration and time, cell viability decreased significantly and significantly compared to control samples. The results of Real-Time PCR showed that the expression of CASPASE-۹ gene at ۴۸ hours increased significantly compared to the control sample. Also, the expression of BAX and BCL۲ genes at ۴۸ and ۷۲ hours increased and decreased significantly compared to the control sample.Conclusion : Rosemary hydroalcoholic extract has anti-cancer effects in breast cancer, can inhibit the growth and proliferation of cancer cells and may be an effective factor in preventing the growth and spread of cancer cells.

Keywords:

Rosmarinus officinalis , BAX , BCL-۲ , CASPASE-۹ , cell line