Antibacterial effects of aqueous extracts of Hypericum perforatum and Myrtus communis on Escherichia coli producing ESBL

Background and Aim : The increasing incidence of infections caused by extended-spectrum beta-lactamase (ESBL) producing Escherichia coli in Iran is of serious concern. The aim of study was to investigate the effect of aqueous hypericum perforatum extract on Escherichia coli producing ESBLs.Methods : ۴۰ strains of Escherichia coli bacteria ESBLS from clinical purified samples were taken from Arak medical university microbial research center infectious diseases and E. coli ATCC۲۵۹۲۲. Antibiogram and Biochemical tests were taken in order to verify Escherichia coli bacteria. The hypericum perforatum plant is prepared freshly from farm herb. Before drying plant in a traditional way, it should be washed with water for several times, hypericum perforatum aqueous extracts were excavated by means of reflux device with distillation and investigating the antibacterial effects of hypericum by disk diffusion method, minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC).Results : Result showed that hypericum perforatum extract to Escherichia coli ESBLs in the concentrations of ۸, ۴ and ۲ mg/ml, ۵۷.۵ percentages of bacteria out of %۱۰۰ have halo and ۴۲.۵ percentages of bacteria out of %۱۰۰ are non-halo. In these concentrations ۱, .۵, .۲۵, .۱۲۵, .۰۶۲۵, .۰۳۱۲, .۰۱۵۶ mg/ml, the percentage of sensitive bacteria (having halo) is decreased and the percentage of resistant bacteria (non-halo) increased. MIC against Escherichia coli taken in different concentrations, which concentration ۰.۰۰۷۸mg/ml was lowest among them.Conclusion : The findings demonstrate that by making a suitable herbal medicine, it is possible to hope for the treatment of resistant infections.

Keywords:

hypericum perforatum , E. coli , Drug resistance , ESBL