Investigating the Rate of Antibiotic-Resistant Bacteria in Positive Cultures

Background and Aim : Microbiological cultures of different specimens such as urine, stool, blood, tissue and wound in for detection of infection and antibiotics is common methods in laboratory. Antimicrobial resistance has been as a threat to the public health. Laboratory had a important role in detection of infection and antimicrobial resistance. The aim of this study was to detection of positive culture and antimicrobial resistance in Besat hospital.Methods : This report was performed in Besat hospital, Kurdistan province, Sanandaj city (Spring ۲۰۲۰). Common cultures specimen’s and laboratory microbiological methods were performed. Disk diffusion also was applied for detection of antibiotics resistance. For statistical analysis, SPSS ۲۰ software, Chi-square test and t-test analysis of variance were used (p≤۰.۰۵).Results : ۵۹۸ Microbiological cultures of different specimens (urine: ۶۰.۳۶%, blood: ۳۱.۴۳%, stool: ۶.۰۲%, wound: ۲.۱%) were performed. ۱۰۱ positive culture were detected (urine: ۳۸.۶۱%, blood: ۵۶.۴۳%, stool: ۰, wound: ۱.۹۸%). ۲۲ of positive culture were defined as positive antibiotics resistance, ۱۳ (۵۹.۰۹%) Extended-spectrum beta-lactamases (ESBL), ۶ (۲۷.۲۷%) Methicillin-resistant Staphylococcus aureus (MRSA), ۳ (۱۳.۶۳%) multidrug resistant (MDR) were detected. No vancomycin-resistant Staphylococcus (VRSA) was detected. It was directly related between number of positive culture and antibiotic resistance (p?۰.۰۵).Conclusion : High antibiotic resistance in bacteria observed. The importance of testing antibiotic sensitivity before prescribing the drug in the treatment and control of drug resistance should be performed.

Keywords:

Antibiotic-Resistant , Positive Cultures , Bacteria