Antibacterial effect of Datura stramoniums ethanolic extraction on the Bacillus subtilis

Background and Aim : Bacillus subtilis is associated with bacteremia/septicemia, endocarditis, meningitis, and infections of wounds, ears, eyes, respiratory, urinary and gastrointestinal tract. The purpose of the present study was to determine of antibacterial effect of Datura stramoniums alcoholic extract on the Bacillus subtilis.Methods : Leaves and bark of the stem of the Datura stramoniums were collected from Behshahr city (Mazandaran province, ۲۰۱۵ summer). Ethanolic extraction was performed by rotary evaporator. Bacillus subtilis ATCC ۶۶۳۳ was applied. Antimicrobial effects by tubes in the concentrations of ۰.۷۸, ۱.۵۶, ۳.۱۲, ۶.۲۵, ۱۲.۵, ۲۵, ۵۰ and ۱۰۰ mg/ml of extract were used. Then disk diffusion, MIC (Minimum Inhibitory Concentration) and MBC (Minimum Bactericidal Concentration) of extracts were determined (Positive control; Gentamicin ۳۰ μg, Negative control: dimethyl sulfoxide). For statistical analysis, SPSS ۱۶ software and two-way analysis of variance were used (p≤۰.۰۵).Results : In the disk diffusion, maximum diameter of the halo was ۱۱.۸ mm at the ۲۰ μl of ۱۰۰ mg/ml of extract. MIC and MBC also were in the ۲۵ mg/ml and ۵۰ mg/ml, respectively. Statistical results showed that increasing the concentration increased antimicrobial effects. It was directly related to the high concentrations of the extract (p?۰.۰۵).Conclusion : The extracts studied have antibacterial effects on Bacillus subtilis. It can be hoped that in the future, by replacing these extracts with antimicrobial drugs. Chemistry drugs, which have always many side effects, so instead of it, herbal medicines can be used in the pharmaceutical industry for infection treatments.

Keywords:

Antibacterial effect , Datura stramoniums , alcoholic extract , Bacillus subtilis