Isolation and Identification of Native Decolourizative Microorganisms from Isfahan Textile Industrial Effluent

Background and Aim : Azo disperse dying are the most widely used among dying in the textile industry. Due to the xenobiotic is one of the most important reasons for water pollution and the environment. They destroy the ecosystem of microorganisms. In addition, it increases the COD of water. In this study, it has been attempted to remove these compounds by native microorganisms isolated from Isfahan textile industrial effluent and the COD parameter is reduced.Methods : In order to isolation of native industrial effluent microorganisms, sampling of textile effluent was done from Sepahan factory in Isfahan city. Accordingly, ۱۸ strains of bacteria were isolated. To decolorization assay was done by UV-Visible spectrophotometry was evaluated along ۱۴۴ h and potent strains were selected. Identification of macroscopic and microscopic was studied followed by biochemical tests. molecular identification done by ۱۶SrRNA primer and the optimization of different concentrations of dying, temperatures and pH were done.Results : Evaluation of the results of blast sequencing revealed ۹۹% similarity to Bacillus cereus. Decolorization assay showed that the green color of the effluent is deleted about ۵۶.۱۲% in ۴۸ hours. The orange color of the effluent was removed at ۴۱.۸۲% along ۴۸ hours. Results of optimizations determined isolated strain have best effect at ۳۰°C and pH ۷. It could reduce ۸۰% COD.Conclusion : Textile industries use several colors that increase the toxicity of these wastewaters, this project's try to use microorganisms to reduce pollution factors (CODs) is a cost-effective investment that will deliver high returns.

Keywords:

Bioremediation , Decolorization , COD , Textile industrial effluent