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Paper
Title

PCR Detection of Herpes Simplex Virus II in Idiopathic Abortions

Year: 1399
COI: MEDISM21_334
Language: EnglishView: 29
نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد

Authors

Sama Babajani ahmadsaraei - Department of biology –Faculty of science, East Tehran Branch Islamic Azad University –Tehran / Iran
Mohammad Hassan shahhosseiny - Department of Microbiology – Shahr-e-Qods Branch – Islamic Azad University –Tehran / Iran
Elaheh Aliasgari - Department of biology –Faculty of science, East Tehran Branch Islamic Azad University –Tehran / Iran

Abstract:

Background and Aim : Herpes simplex virus type ۲ (HSV-۲) is one of the most important human pathogenic viruses. The virus causes genital herpes and can cause miscarriage, pregnancy complications, and infertility in women. The aim of this study was to evaluate the role of HSV-۲ in idiopathic abortion by PCR.Methods : Fifty blood serum samples of women with idiopathic miscarriages were collected and transferred to the laboratory. The PCR test was performed using standard HSV-۲ strain DNA and then the limit of detection (LOD) and specificity of the test were determined using different strain of DNA. Standard test was performed using positive and negative controls on the samples and the results were analyzed by agarose gel electrophoresis.Results : PCR test optimized and ۲۳۱ bp amplicon were observed in agarose gel. In specificity test only with DNA HSV-۲ achieved positive results as well as a limit of detection ۱۰ Copy / Reaction. Of the ۵۰ samples studied, ۹ (۴.۵%) were positive.Conclusion : Idiopathic miscarriages are miscarriages that do not seem to have a definite cause. But clearly, several factors, including infectious agents that are difficult to identify, can cause miscarriage. One of these factors could be HSV-۲. The results of this study showed that HSV-۲ is one of the most important factors in this type of miscarriage and that HSV-۲ can be quickly identified by tests such as PCR.

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Paper COI Code

This Paper COI Code is MEDISM21_334. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/1255010/

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Babajani ahmadsaraei, Sama and shahhosseiny, Mohammad Hassan and Aliasgari, Elaheh,1399,PCR Detection of Herpes Simplex Virus II in Idiopathic Abortions,21th International Congress of Microbiology of Iran,Tehran,https://civilica.com/doc/1255010

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Scientometrics

The specifications of the publisher center of this Paper are as follows:
Type of center: Azad University
Paper count: 1,441
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