Screening of tyrosinase enzyme producing Actinobacterial isolates from soil samples of Iran

Background and Aim : Tyrosinases are one of the most important enzymes in biotechnology and play a special role in various industries, including bioremediation industries. Although various natural sources are considered to find tyrosinase, the enzymes derived from microbial sources, in particular bacterial sources, have practical applications and more studied. Actinobacteria are potent bacteria in biotechnology and are used to produce various metabolites with therapeutic, industrial and environmental applications.Methods : . In this research, ۲۰ soil samples were obtained from different regions of Iran, cultured in ISP۲ agar and ۵۸ actinobacterial strains were isolated. The isolates were cultured in tyrosine agar plates and ۱۷ strains with transparent halo were considered as tyrosinase producers. These isolates were cultured in tyrosine broth and the tyrosinase activity was confirmed in ۱۱ strains.Results : The results of the quantitative evaluation of tyrosinase activity on the top ۴ strains showed that the two strains designated ۳۱۶۵ and ۳۲۳۳ had more tyrosinase activity. According molecular identification, these isolates are ۹/۹۹% similarity in۱۶ SrRNA gene sequencing to Streptomyces thermocarboxydus and Streptomyces tendae.Conclusion : The results of this study indicated the prevalence of tyrosinase producing actinobacteria in the soils of Iran.

Keywords:

tyrosinase enzyme , actinobacteria , Streptomyces , primary screening , secondary screening , molecular identification