Investigation on the impact of pesticides on microbial flora of saline soils using the next generation sequencing

Background and Aim : Pesticides and insecticides are the chemicals widely used in the agricultural industry, but their ecotoxicological effects on the environment is not still well understood. In this study, the impact of a pesticide, chlorpyrifos (CP) and an insecticide, deltamethrin (DM) as the organophosphorus and pyrethroid compounds on soil microbial diversity was investigated .Methods : four different soils samples under the various salinity (۰, ۱%, ۲% and ۴%) were contaminated by these compounds. Then, the destructive effects of CP and DM on the soil microbial composition were studied by next generation sequencing (NGS).Results : The results indicated that soil salinity had significant impact on the sample's resistance to the toxins and some of the dominant species in the blank sample were completely destroyed by CP and DM.At the phylum level ,Firmicutes and Proteobacteria were not be destroyed by DM and CP, but their population also were considerably raised by degrading the contaminantsConclusion : The present results provided a momentous insight into the environmental impacts of chlorpyrifos and deltamethrin contaminants and their natural attenuation.

Keywords:

Pesticides , deltamethrin , chlorpyrifos , next generation sequencing , Soil Salinity