Evaluation of the effect of ethanolic extract of Reum Lribes on the expression of Caspase۳ gene in MCF_۷ breast cancer cell line

Background and Aim : : Problems such as drug resistance, high cost of treatment, and some problems with cancer treatment have led many researchers to turn to medicinal plants in relation to cancer, because medicinal plants have fewer side effects compared to chemical drugs. They are also relatively inexpensive to treat with herbs. Studies have shown that black seed has anti-cancer effects, but the molecular mechanism of its effect on cell death in cancer cells is unclear. Therefore, in the present study, the effect of ethanolic extract of Reum Lribes plant on cell survival rate of MCF۷ and expression of Caspase ۳ gene was investigated.Methods : In this laboratory study, the effect of ethanolic extract of Reum Lribes plant on survival and expression of Caspase ۳ gene in breast cancer cells MCF_۷ was investigated. For this purpose, MFC-۷ breast cancer cells were cultured and treated with ethanolic extract of Reum Lribes plant with different concentrations for ۲۴, ۴۸ and ۷۲ hours. The effect of the extract on cell viability was assessed by MTT. RNA extraction was performed and cDNA was performed to measure the expression of Caspase ۳ gene Real Time PCR.Results : This study showed that with increasing concentration and time, cell viability decreased significantly and significantly compared to control samples. Also, the results of Real-Time PCR showed that the expression of Caspase ۳ gene at ۴۸ and ۷۲ hours increased significantly compared to the control sample.Conclusion : It seems that using the medicinal plant Reum Lribes, the expression of Caspase ۳ gene is also increased, which causes the death of cancer cells.

Keywords:

Breast cancer , Reum Lribes , Caspase۳ gene