Fernanda Dias - Department of Veterinary Medicine, Universidade de Franca, Franca (SP), Brazil
Lucas Pereira - Department of Veterinary Medicine, Universidade de Franca, Franca (SP), Brazil
Ricardo Furtado - Department of Veterinary Medicine, Universidade de Franca, Franca (SP), Brazil
Geórgia Modé Magalhães - Department of Veterinary Medicine, Instituto Federal do Sul de Minas Gerais, Muzambinho (MG), Brazil
Marina Miguel - Department of Veterinary Medicine, Universidade Federal de Goiás, Goiânia (GO), Brazil
Luis Gustavo Dias - Department of Veterinary Medicine, Universidade Estadual Paulista, Jaboticabal (SP), Brazil
Adriana Jorge - Department of Veterinary Medicine, Universidade de Franca, Franca (SP), Brazil
Cristiane Honsho - Department of Veterinary Medicine, Universidade de Franca, Franca (SP), Brazil
Sérgio Ambrósio - Department of Sciences, Universidade de Franca, Franca (SP), Brazil
Jairo Bastos - Department of Pharmacy, Universidade de São Paulo, Ribeirão Preto (SP), Brazil
Micaela Carrijo - Department of Veterinary Medicine, Universidade de Franca, Franca (SP), Brazil
Denise Tavares - Department of Sciences, Universidade de Franca, Franca (SP), Brazil

Objective: Evaluation of the healing and toxicological effects of Copaifera duckei Dwyer oleoresin (CDO). Materials and Methods: Rodents with skin lesions were divided into nine groups, including daily treatments with ۱, ۳ and ۱۰% CDO, collagenase, antibiotic ointment and control groups, for ۱۴ days. Results: Treatment with ۱۰% CDO reduced skin edema and hyperplasia, demonstrating anti-inflammatory effect of the oil. Reduction in the wound area was observed, indicating the healing effect of CDO. Histopathological analysis showed increases in angiogenesis and re-epithelialization in animals treated with the highest concentration. On the other hand, no alterations in ulcerations, inflammatory infiltrate, hemorrhage, congestion, degeneration, percentage of collagen fibers, number of cells stained with anti-macrophage migration inhibitory factor, or density of area stained with anti-collagen I and III were found. Toxicogenetic analysis revealed no differences in micronucleus frequencies or in the ratio of polychromatic erythrocytes to total erythrocytes between treated and negative control, demonstrating the absence of genotoxicity and cytotoxicity, respectively. There was no difference in levels of liver enzymes among groups, indicating the absence of hepatotoxicity. Conclusion: Formulations of CDO exerted beneficial effects on the stages of cutaneous wound healing and are promising options for the treatment of wounds.  

Cutaneous wound healing, Copaifera duckei Dwyer oleoresin, Toxicity