Faezeh Moradi Negahdari - Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran
Mousa-Al-Reza Hadjzadeh - Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Zahra Gholamnezhad - Applied Biomedical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Zahra Samadi Noshahr - Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Zakieh Keshavarzi - Department of physiology, North Khorasan University of Medical Sciences, Bojnurd, Iran

Objective: The effect of trans-anethole and metformin on biochemical and hormonal changes of testosterone-induced Polycystic ovary syndrome (PCOS) in rats was investigated. Materials and Methods: Female Wister rats (n=۴۸) were randomly divided into six groups: control; PCOS; PCOS+metformin (۳۰۰ mg/kg); and PCOS+trans-anethole (۲۰, ۴۰, and ۸۰ mg/kg). PCOS was induced by intraperitoneal injection of testosterone (۱ mg/kg/day) for ۳۵ days. After induction of PCOS, trans-anethole and metformin were given orally for ۳۰ days. Finally, blood sugar, insulin, lipid profile, and testosterone and dehydroepiandrosterone (DHEAS) as well as animals’ weight, and water and food intake were determined. Results: In all treated and untreated PCOS groups, serum testosterone levels were significantly increased compared to the control group (p Conclusion: These results showed that trans-anethole significantly decreased serum levels of insulin, DHEAS and blood lipids. It can be concluded that trans-anethole ameliorates PCOS biochemical and hormonal change in PCOS rats; therefore, it might be suggested as a beneficial remedy for further clinical evaluations in PCOS patients.

PCOS, Testosterone, Trans-anethole, Metformin, Insulin, Dehydroepiandrosterone