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Title

Extremely low frequency electromagnetic fields change BCL۲,miR-۱۵-b and miR-۱۶ expression in human gastric cancer cell line

Year: 1400
COI: BCBCN04_057
Language: EnglishView: 64
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Authors

Sahar heidari - Graduate student of Biology, Department of Biology, Science and research Branch, Islamic Azad University, Tehran, Iran.
soheila abdi - Assistant Professor, Department of Physics, Safadasht Branch, Islamic Azad University, Tehran, Iran.
Shohreh Zare Karizi - Assistant Professor, Department of Genetics, School of Biological Sciences, Varamin-Pishva Branch, Islamic Azad university, Varamin, Iran.

Abstract:

In this research, changes in the expression of BCL۲, miR-۱۵-b, and miR-۱۶ in human gastric cell line (AGS) following the exposure to magnetic flux densities (MFDs) of ۰.۲ and ۲ mT for ۱۸ h were investigated. Changes in the cell viability were evaluated by MTT assay. Real-time PCR was used to evaluate the expression changes of BCL۲, miR-۱۵-b, and miR-۱۶. The results showed that ELF-EMF could significantly reduce the viability of AGS cells in the continuous MFD of ۲ mT. The BCL۲ expression was significantly decreased following the exposure to continuous MFDs of ۰.۲ and ۲ mT and discontinuous MFD of ۲ mT. The expression of miR-۱۵-b, and miR-۱۶ was significantly increased in continuous and discontinuous MFD of ۲ mT. According to the results, weak and moderate ELF-EMFs can change the expression of BCL۲, miR-۱۵-b, and miR-۱۶.

Keywords:

Electromagnetic fields , Adenocarcinoma gastric cell line (AGS) , MiR-۱۵-b , miR۱۶ , BCL۲

Paper COI Code

This Paper COI Code is BCBCN04_057. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/1259688/

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heidari, Sahar and abdi, soheila and Zare Karizi, Shohreh,1400,Extremely low frequency electromagnetic fields change BCL۲,miR-۱۵-b and miR-۱۶ expression in human gastric cancer cell line,Fourth National Conference on Innovation and Technology of Biological Sciences, Iranian Chemistry,Tehran,https://civilica.com/doc/1259688

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Type of center: Azad University
Paper count: 33,902
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