Marzieh Moheb Alian - Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Fatemeh Khakpai - Assistant Professor of Cognitive and Neuroscience Research Center (CNRC), Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Neda Mosavi Niri - Department of Medical Biotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Maryam Naseroelslami - Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

Diabetes is on the rise in communities. So far, no drug has been identified that can completely cure the disease. Because natural compounds have fewer side effects than chemical compounds, treatment with these methods .Therefore, the aim of this study was to treat diabetes using niosome stevia in a model of male diabetic rats.The purpose of this study was to evaluate the effect of niosome nanoparticles encapsulating stevia extract on the expression of Glut۲ and Glut۹ genes in type ۲ diabetic model induced by intravenous injection of streptozotocin in male rats.In this study, ۴۰ Wistar rats with an average weight of ۲۰۰-۲۵۰ g were used. They were divided into ۵ groups of ۸, including healthy control, diabetic control (sham), stevia, niosome stevia, niosome. After becoming diabetic, mice received daily stevia and stevia niosome and niosome at a dose of ۵۰۰ mg / kg by gavage for ۳۰ days. Forty-eight hours after the last treatment, rats were anesthetized and liver tissue was removed from their bodies, and real-time PCR was performed to examine the glut ۲ and glut ۹ genes.Streptozotocin injection caused a significant increase in blood sugar compared to the control group (p <۰.۰۰۱). Glut۲ gene expression was increased in the stevia and stevia nanoniosome groups compared to the control and sham groups. (p <۰.۰۰۱) The expression of glut۹ gene was increased in stevia and stevia nanoniosome groups (p <۰.۰۰۱).Due to the fact that in this study, the expression of glut ۲ and glut ۹ genes had increased compared to the diabetic group due to treatment, and this increase was more in the niosome group. It can be said that one of the effects of stevia in improving diabetes is the effect on the expression of genes involved in glucose transfer. So we can say that by updating the drug, we can increase its stability and efficiency in the treatment of diabetes.

Diabetes , Stevia , GLUT۲ gene , GLUT۹ gene ,Niosome