Evaluation of IL-۲۳ Gene expression in cardiovascular patients and its comparison with coronary artery disease

Atherosclerosis is a chronic inflammatory disease of the walls of blood vessels that involves both innate and acquired immune systems . The role of IL-۲۳ in inflammatory diseases, autoimmunity and coronary artery disease has been considered in recent years.The aim of this study was to evaluate the expression of inflammatory cytokine IL-۲۳ in peripheral blood lymphocytes (PBMCs) in patients with coronary artery disease .In this study, after collecting blood samples, patients with more than ۵۰% coronary artery occlusion were classified as the study group and patients without coronary artery stenosis as the control group .Then RNA isolation as well as cDna fabrication was performed for all groups and finally the expression level of IL-۲۳ gene was quantitatively examined by Real Time PCR .The findings of this study showed that there was no statistically significant difference between IL-۲۳ gene expression and coronary artery pathogenesis (CI = ۰.۳۹-۰.۳, P = ۰.۸۱۲) .It should be noted that the results in both tests were obtained from diabetic and non-diabetic people, people with high blood pressure and people without blood pressure at a value of P value> ۰.۰۵ .Statistical data in this study show that the expression of IL-۲۳ gene did not have a significant effect on the pathogenicity of people with coronary artery disease, while it was previously shown that the expression of IL-۲۳ gene had a significant role in this disease .These results indicate that IL-۲۳ can be used for therapeutic applications in such diseases.

Keywords:

Atherosclerosis , Real time PCR , Diabetes , Blood pressure